METHOD FOR DIFFERENTIATION OF TULAREMIA MICROBIAL SUBSPECIES Russian patent published in 2013 - IPC C12Q1/68 C12N15/31 

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to biotechnology, namely a method for differentiation of tularemia microbial subspecies. The invention may be used in laboratory diagnosis of tularemia. The method involves PCR amplification with the use of gene-specific iglC and chromosome regions containing E. coli-like Chi-sequences of the primers FiglC-AAGGATAAGACCTGTCTG, RiglC-TTGAAACCATACCGGGTA and Chi If CTAGG-GCTGGTGG-G. It is followed by electrophoresis of the amplification products to be differentiated by comparing electrophoretic mobility of the produced amplicons and mobility of DNA marker fragments. If observing a specific light-producing strip at the level of 986 base pairs, the strain data are recorded as Francisella gen. The strip distribution pattern within the range of ~190 to ~830 base pairs enables differentiating the analysed samples at the level of tularemia microbial subspecies: ~190 base pairs for subsp. novicida, ~500-570 base pairs for subsp. mediasiatica, ~570 base pairs for subsp. holarctica, whereas subsp. tularensis is characterized by the fragment of ~500 base pairs.

EFFECT: presented invention enables accurate, instant and high-specific method for differentiation of F tularensis subspecies.

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