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2 671 099

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(51)

IPC

C12N15/00(2006-01-01)

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Application

2017127664, 2017-08-01

(24)

Start date

2017-08-01

(22)

Actual filing date

2017-08-01

(45)

Published

2018-10-29

(72)

Inventor

Monakhova Elena VladimirovnaPisanov Ruslan VyacheslavovichDemidova Galina ViktorovnaNepomnyashchaya Natalya Borisovna

(73)

Holder

Federalnoe Kazennoe Uchrezhdenie Zdravookhraneniya Ordena Trudovogo Krasnogo Znameni Nauchno-Issledovatelskij Protivochumnyj Institut" Federalnoj Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchiya Cheloveka

RECOMBINANT PLASMID EXPRESSING CLONED GENE OF VIBRIO CHOLERAE HEMOLYSIN AND ESCHERICHIA COLI - VIBRIO CHOLERAE PRO-HEMOLYSIN SUPERPRODUCENT Russian patent published in 2018 - IPC C12N15/00

Abstract RU 2671099 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology, in particular to the recombinant plasmid pHlyA. Plasmid pHlyA expresses the cloned hlyA (hemolysin) gene of Vibrio cholerae, embedded at the BamHI-Pstl sites in the polylinker of the vector plasmid pQE30 under the control of the T5 promoter. Invention also relates to the strain of Escherichia coli KM 2028. This strain is the carrier of the plasmid pHlyA and is intended for the production of pro-hemolysin (proHlyA) of Vibrio cholerae.

EFFECT: present invention makes it possible to obtain pro-hemolysin in high yield.

2 cl, 2 dwg, 3 ex

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RU 2 671 099 C1

Authors

Monakhova Elena Vladimirovna

Pisanov Ruslan Vyacheslavovich

Demidova Galina Viktorovna

Nepomnyashchaya Natalya Borisovna

Dates

2018-10-29—Published

2017-08-01—Filed