FIELD: biotechnology.
SUBSTANCE: method of highly effective production of methionine-containing form of staphylokinase (SAK-1) and some of its biologically active analogs in a host cell, providing culturing a bacterial cell transformed by the expression vector which comprises the DNA sequence encoding SAK-1 at a high degree of aeration and reducing the level of oxygen by 5% of atmospheric level with achievement the exponential growth phase.
EFFECT: use of the invention provides a significant increase in the yield of the target product.
11 dwg, 4 ex
| Title |
Year |
Author |
Number |
| METHOD OF PRODUCING RECOMBINANT STAPHYLOKINASE WITH REGULATION OF OXYGEN LEVEL |
2004 |
- Radzhamokhan Govindan
- Dakhija Monika
- Patkhania Randzhana
- Dikshit Kanak Lata
|
RU2373281C2 |
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2009 |
- Vil'Kin Jakov Fimovich
- Markin Sergej Sergeevich
- Semenov Andrej Mikhajlovich
|
RU2422522C2 |
| RECOMBINANT PLASMID DNA CONTAINING SEQUENCE OF MATURE STAPHYLOKINASE STAPHYLOCOCCUS AUREUS WITH REPLACEMENT OF CODONS K74, E75 AND R77 WITH TRIPLETS CODING Ala, STRAINS OF ESCHERICHIA COLI MZ09 AND METHOD OF OBTAINING RECOMBINANT PROTEIN, CONTAINING SEQUENCE OF MATURE STAPHYLOKINASE GENE WITH REPLACEMENT OF CODONS K74, E75 AND R77 WITH Ala-CODING TRIPLETS |
2010 |
- Vil'Kin Jakov Fimovich
- Markin Sergej Sergeevich
- Semenov Andrej Mikhajlovich
|
RU2422153C1 |
| RECOMBINANT PLASMID DNA ENCODING COMPLETE SEQUENCE OF STAPHYLOKINASE, STRAIN OF ESCHERICHIA COLI SA 9325 AS PRODUCER OF RECOMBINANT PROTEIN |
1999 |
- Markin S.S.(Ru)
- Spizhenko Jurij Prokof'Evich
- Markov A.P.(Ru)
- Nechaeva E.V.(Ru)
- Ljashenko A.A.(Ru)
- Uvarov V.Ju.(Ru)
- Tarasov Aleksandr Andreevich
- Zhukova A.V.(Ru)
- Semenov M.P.(Ru)
|
RU2156299C1 |
| RING DNA MOLECULE WITH CONDITION-DEPENDENT REPLICON ORIGIN, METHOD FOR PRODUCTION THEREOF AND USES IN GENE THERAPY |
2003 |
|
RU2307870C2 |
| RECOMBINANT STAPHYLOKINASE DERIVATIVE, METHOD FOR PRODUCTION AND USES THEREOF |
2001 |
|
RU2283863C2 |
| OBTAINING XYLITOL FROM GLUCOSE BY MEANS OF A RECOMBINANT STRAIN |
2015 |
- Gerard, Tania
- Heysen, Arnaud
- Thor, Friederike
- Defretin, Sophie
- Schaefer, Astrid
- Diefenbacher, Melanie
- Chang, Yiming
- Honda Malca, Sumire
- Schwab, Markus
|
RU2693593C2 |
| DICISTRONIC BACTERIAL EXPRESSION SYSTEM |
2015 |
- Salunkhe, Shardul
- Varshney, Brajesh
- Soorapaneni, Sudheerbabu
|
RU2732148C2 |
| FUSED PROTEIN OF TELOMERASE REVERSE TRANSCRIPTASE, NUCLEOTIDES CODING THEM, AND USING THEM |
2007 |
- Chiliberto Dzhennaro
- La Monika Nikola
- Mennuni Karmela
- Skarselli Ehliza
|
RU2473691C2 |
| PLASMID FOR EXPRESSION IN BACTERIA CELLS OF GENUS Escherichia OF PRECURSOR OF RECOMBINANT FRAGMENT OF HUMAN TISSUE PLASMINOGEN ACTIVATOR (TPA) (RETEPLASE), BACTERIA BELONGING TO GENUS Escherichia, - PRODUCER OF PRECURSOR OF RECOMBINANT FRAGMENT OF HUMAN TPA (RETEPLASE) OR [-1] METHIONYL - FRAGMENT OF HUMAN TPA (RETEPLASE), PRECURSOR OF RECOMBINANT FRAGMENT OF HUMAN TPA (RETEPLASE), METHOD OF PRODUCTION OF PRECURSOR OF RECOMBINANT FRAGMENT OF HUMAN TPA (RETEPLASE) |
2011 |
- Orlova Nadezhda Aleksandrovna
- Vorob'Ev Ivan Ivanovich
|
RU2495933C2 |
