RECOMBINANT PLASMID DNA CONTAINING SEQUENCE OF MATURE STAPHYLOKINASE STAPHYLOCOCCUS AUREUS WITH REPLACEMENT OF CODONS K74, E75 AND R77 WITH TRIPLETS CODING Ala, STRAINS OF ESCHERICHIA COLI MZ09 AND METHOD OF OBTAINING RECOMBINANT PROTEIN, CONTAINING SEQUENCE OF MATURE STAPHYLOKINASE GENE WITH REPLACEMENT OF CODONS K74, E75 AND R77 WITH Ala-CODING TRIPLETS Russian patent published in 2011 - IPC A61K38/49 C12N15/58 C12N15/70 C12N9/12 C12N1/21 A61P7/02 

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to field of biotechnology and deals with recombinant plasmid DNA, which contains sequence of gene of mature staphylokinase Staphylococcus aureus with replacement of codons K74, E75 and R77 with triplets, which code Ala, of strain Escherichia coli MZ09 and method of obtaining recombinant protein, which contains sequence of gene of mature staphylokinase with replacement of codons K74, E75 and R77 with Ala-coding triplets. Essence of method includes recombinant plasmid DNA, which codes sequence of gene of mature protein of staphylokinase from Staphylococcus aureus staphylokinase with replacement of codons K74, E75 and R77 with Ala-coding triplets, which has nucleotide sequence, given in dwg.1. Invention also includes strain Esherichia coli MZ09, producent of recombinant staphylokinase, with sequence, given in dwg.1, as well as method of its obtaining.

EFFECT: invention makes it possible to obtain staphylokinase protein which possesses high fibrinogenic activity, with output of recombinant protein to 20% from total amount.

3 cl, 2 dwg