FIELD: chemistry.
SUBSTANCE: invention relates to a method of producing 2S canola protein, involving solubilisation of canola proteins from canola oil seed flour using 0.25-0.35M sodium chloride solution with pH 5-6 to form a canola protein solution. The canola protein solution is separated from the residual canola oil seed flour. The canola protein solution is brought into contact with a cation-exchange medium at pH 5-6 to bind 2S canola protein to the cation-exchange medium in preference to other canola proteins. The bound 2S canola protein is separated from unbound canola proteins and impurities, and the bound 2S canola protein is extracted from the cation-exchange medium using 0.55-0.7M aqueous sodium chloride solution.
EFFECT: method enables to obtain 2S canola protein with purity of 99,9%.
6 cl, 9 tbl, 2 ex
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Authors
Dates
2013-08-20—Published
2008-08-01—Filed