POLYNUCLEOTIDE PRIMERS Russian patent published in 2013 - IPC C07H21/00 C12Q1/68 

FIELD: chemistry.

SUBSTANCE: invention relates to field of biotechnology, namely to method and set for determining presence or absence of mutation in gene PIK3CA. Method includes mixing sample of nucleic acid, which contains at least fragment of gene P1K3CA, containing at least one mutation, selected from E542K, E545K, H1047R and H1047L, with polynucleotide primer, which consists of SEQ ID NO:5, SEQ ID NO:14, SEQ ID NO:21 or SEQ ID NO:28, where mixture also contains second primer, and second primer corresponds to area of fragment of sequence PIK3CA, located lower than area, which is complementary to polynucleotide, and carrying out PCR of the mixture. Hybridisation of polynucleotide with sample of nucleic acid is detected, where hybridisation indicates presence of mutation. Set includes at least two primers, where first primer consists of SEQ ID NO:5, SEQ ID NO:14, SEQ ID NO:21 or SEQ ID NO:28, and second primer corresponds to area of fragment of sequence PIK3CA, located lower than area, which is complementary to polynucleotide.

EFFECT: claimed invention makes it possible to efficiently detect mutations in fragments of gene PIK3CA, which includes at least one mutation, selected from E542K, E545K, H1047R and H1047L.

4 cl, 1 dwg, 4 tbl, 4 ex