FIELD: biotechnologies.
SUBSTANCE: method to protect yeast Saccharomyces cerevisiae against oxidising stress as a result of exposure to hydrogen peroxide includes growing of yeast culture under standard conditions till the end of the logarithmic or the start of the stationary phase of growth, incubation with a protective agent, exposure to hydrogen peroxide with subsequent determination of the number of survived cells. The protective agent is represented by soy inhibitors of proteases in concentration of 0.1-2.0 g/l. The time of incubation with the protective agent makes 5-6 hr, concentration of hydrogen peroxide is 700-750 mM. The invention provides for high extent of yeast cells production with no depressant action.
EFFECT: share of survived yeast cells during method realisation increases in comparison with the reference one 1,2-5,4 times.
7 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD OF PRODUCING LACTIC ACID | 2019 |
|
RU2712703C1 |
METHOD FOR PRODUCING SEED YEASTS | 2006 |
|
RU2329300C1 |
METHOD FOR CULTIVATION OF YEAST FOR ALCOHOL PRODUCTION | 2007 |
|
RU2394098C2 |
METHOD FOR PRODUCTION OF FODDER YEAST BIOMASS | 2008 |
|
RU2393719C2 |
METHOD OF OBTAINING BIOMASS OF CAROTENE-SYNTHESISING MICROORGANISMS | 2012 |
|
RU2553213C2 |
GLUTATHIONE-PRODUCING YEAST STRAIN AND A METHOD OF PRODUCING GLUTATHIONE USING THE SAME | 2020 |
|
RU2800992C1 |
ELASTASE INHIBITOR | 2010 |
|
RU2548794C2 |
FIBROBLAST GROWTH AGENT | 2009 |
|
RU2492850C2 |
METHOD FOR PRODUCTION OF PROTEIN-HYDROCARBON BIOLOGICALLY ACTIVE FODDER ADDITIVE | 2008 |
|
RU2391857C2 |
METHOD FOR PREPARING YEAST BIOMASS | 2004 |
|
RU2268924C1 |
Authors
Dates
2013-09-20—Published
2012-02-27—Filed