FIELD: biotechnologies.
SUBSTANCE: method includes cultivation of a fungus of Fusarium sambucinum type on nutrient medium containing a source of carbon 3-4%, nitrogen 0.2-0.3%, phosphorus 0.2-0.3% and microelements 0.07-0.08%, under sterile conditions at temperature of 26-30°C with mixing and aeration within 36-72 hours with subsequent separation of the cultural liquid from fungus biomass. Besides, the cultural fluid is centrifuged at room temperature for 15-20 min. at 800-1500 rpm, and the target product is made from supernatant by separation of peptides with molecular mass from 3,000-60,000 D. Also before centrifugation the cultural fluid is heated up to 110-115°C and pressure of 1.3-1.5 atm. with rate of 1.5-2 degrees/min. with the following exposure for 1.5-2.5 hours.
EFFECT: adjuvant prepared in accordance with the invention has higher potency that stimulates immunity, is harmless within vaccines and other products of immunological purpose, areactogenic, may be applied both independently and in combination with oil adjuvants or adjuvants-sorbents on the basis of aluminium hydroxide gel and other substances.
2 cl, 3 tbl, 9 ex
