FIELD: biotechnologies.
SUBSTANCE: host cell Trichoderma reesei contains polynucleotides that code a heterologous polypeptide GH61, fusion protein of beta-glucosidase, cellulolytic ferments. The method to produce the composition includes cultivation of the host cell Trichoderma reesei and extraction of the composition. The composition conatins the polypeptide GH61, fusion protein of beta-glucosidase and one or more cellulolytic ferments. The method to decompose a material that contains cellulose is characterised by treatment of the material with a cellulolytic protein composition. The method to produce a fermentation product includes saccharification of the material, fermentation of the saccharified material with fermenting microorganisms and extraction of fermentation products.
EFFECT: presented inventions may be used for decomposition or transformation of a cellulose-containing material.
12 cl, 14 dwg, 23 ex
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