FIELD: chemistry.
SUBSTANCE: fused proteins contain an endoglucanase nucleus amino acid sequence having at least 95% identity to SEQ ID NO:2, fused with an amino acid sequence containing a linker and a cellulose-binding domain (CBD), having at least 95% identity to SEQ ID NO:15. Such fused proteins can be obtained via a recombinant technique using suitable polynucleotides, expression vectors and host cells.
EFFECT: invention provides cellulase, having low activity with respect to restaining, and can be used to treat cellulose material; disclosed fused proteins and enzyme preparations based thereon can be used to prepare detergent compositions or for improving quality of animal feed.
26 cl, 8 dwg, 10 tbl, 10 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF TREATING CELLULOSE MATERIAL AND ENZYMES USED THEREIN | 2006 |
|
RU2458128C2 |
| RECOMBINANT PROTEIN LACspCBD POSSESSING BETA-GALCTOSIDASE ACTIVITY AND ABILITY FOR SPONTANEOUS BINDING WITH CELLULOSE-CONTAINING SORBENTS, RECOMBINANT PLASMID DNA ENCODING SYNTHESIS OF RECOMBINANT PROTEIN LACspCBD, STRAIN Escherichia coli M15 [pREP4, pLACspCBD] AS PRODUCER OF RECOMBINANT PROTEIN LACspCBD, METHOD FOR PREPARING IMMOBILIZED RECOMBINANT PROTEIN LACspCBD ON CELLULOSE AND METHOD FOR ENZYMATIC CLEAVAGE OF LACTOSE | 2004 |
|
RU2278160C2 |
| METHODS FOR IMPROVING INTENSIFYING CELLULOLYTIC ACTIVITY OF POLYPEPTIDE | 2008 |
|
RU2510417C2 |
| POLYPEPTIDES HAVING CELLULASE ACTIVITY | 2010 |
|
RU2597289C2 |
| ENZYME COMPOSITION, CAPABLE OF EFFICIENT DECOMPOSITION OF CELLULOSE MATERIAL | 2009 |
|
RU2529949C2 |
| MASHING METHOD | 2004 |
|
RU2376347C2 |
| NEW EXPRESSION-REGULATING SEQUENCES AND EXPRESSION PRODUCTS IN HYPHAMYCETES REGION | 2001 |
|
RU2272835C2 |
| RECOMBINANT PROTEIN Collbd-CBD, RECOMBINANT PLASMID pOC-Collbd, STRAIN Escherichia coli-PRODUCER OF RECOMBINANT PROTEIN Collbd-CBD, METHOD TO PRODUCE RECOMBINANT PROTEIN Collbd-CBD | 2009 |
|
RU2408726C1 |
| COMPOSITION OF ENZYME MIXTURE FOR HYDROLYSIS OF MIXTURE OF CELLULOSE AND HEMICELLULOSE MATERIALS (VERSIONS) AND METHODS FOR USE THEREOF (VERSIONS) | 2009 |
|
RU2536256C2 |
| GENETIC MAKER TO ENSURE EXPRESSION OF TARGET HOMOLOGOUS AND HETEROLOGOUS GENES IN CELLS OF FILAMENTOUS FUNGUS PENICILLIUM VERRUCULOSUM; USED AS HOST, METHOD FOR MAKING STRAIN OF FUNGUS PENICILLIUM VERRUCULOSUM AND METHOD FOR MAKING ENZYMATIC AGENT | 2008 |
|
RU2378372C2 |
