FIELD: biotechnologies.
SUBSTANCE: genetic structure pAd-SM is produced, being built by homological recombination of the vector pAdEasy-1, containing the main part of genome of adenovirus and plasmid pAdTrack-CMV, where cDNA fragments of human genes SOX2 and C-MYC are placed, being connected with a nucleotide sequence that codes P2A-peptide. The plasmid pAd-SM produced as a result of homological recombination contains a fragment SOX2-P2A-C-MYC under control of a constitutive promotor CMV.
EFFECT: possibility to produce adenovirus particles for simultaneois delivery of genes and expression of proteins SOX2 and C-MYC in human cells, pAd-SM contains a gene that codes a fluorescent protein EGFP, under control of a CMV promotor, which makes it possible to track transduction of cells and elimination of virus DNA from a cell.
3 cl, 1 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT PLASMID pSM-ZsGreen, CODING HUMAN PROTEINS SOX2 AND C-MYC AND FLUORESCENT PROTEIN ZsGreen, INTENDED TO PRODUCE INDUCED PLURIPOTENT HUMAN STEM CELLS | 2011 |
|
RU2477314C1 |
| RECOMBINANT PLASMID pSN-ZsGreen, CODING SOX2 AND NANOG HUMAN PROTEINS AND FLUORESCENT PROTEIN ZsGreen, DESIGNED TO PRODUCE INDUCED PLURIPOTENT HUMAN STEM CELLS | 2011 |
|
RU2495124C2 |
| RECOMBINANT PLASMID POL-DSRED2, CODING OCT4 AND LIN28 HUMAN PROTEINS AND FLUORESCENT PROTEIN DSRED2, DESIGNED TO PRODUCE INDUCED PLURIPOTENT HUMAN STEM CELLS | 2011 |
|
RU2495126C2 |
| RECOMBINANT PLASMID pOK-DsRed2, CODING OCT4 AND KLF4 HUMAN PROTEINS AND FLUORESCENT PROTEIN DsRed2, DESIGNED TO PRODUCE INDUCED PLURIPOTENT HUMAN STEM CELLS | 2011 |
|
RU2495125C2 |
| RECOMBINANT PLASMID DNA pSNCA-C-3XFLAG-2XST-DONOR PROVIDING STABLE DOXYCYCLINE-DRIVEN EXPRESSION OF ALPHA-SYNUCLEIN CHIMERIC PROTEIN IN HUMAN CELL CULTURES | 2022 |
|
RU2795156C1 |
| METHOD OF OBTAINING OF PATIENT (DONOR) - SPECIFIC FIBROBLAST-LIKE CELLS FROM INDUCED PLURIPOTENT HUMAN STEM CELLS | 2014 |
|
RU2568059C1 |
| VECTOR, METHOD FOR TREATING CHOROIDITIS, METHOD FOR SELECTIVE EXPRESSION OF POLYNUCLEOTIDE | 2014 |
|
RU2723101C2 |
| RECOMBINANT PLASMID DNA LENTI sgRNA(MS2)_zeo_Myc, PROVIDING ACTIVATION OF Myc GENE EXPRESSION IN HUMAN CELLS, METHOD OF OBTAINING HUMAN CELLS WITH STABLY INCREASED EXPRESSION OF Myc GENE AND MONOCLONAL HUMAN BREAST CANCER CELL LINE BT549_Myc WITH STABLY INCREASED EXPRESSION OF Myc GENE | 2022 |
|
RU2812975C1 |
| METHOD OF PRODUCING MAMMAL EMBRYONIC STEM CELLS MODIFIED WITH HUMAN NERVE GROWTH FACTOR GENE | 2011 |
|
RU2458984C1 |
| METHOD FOR STIMULATING SPINAL CORD REGENERATION WITH GENETICALLY MODIFIED HUMAN UMBILICAL CORD BLOOD CELLS | 2013 |
|
RU2521225C2 |
