FIELD: medicine.
SUBSTANCE: what is described is a method for preparing sample for gas chromatographic urine thioglycolic acid test involving derivatisation, ethyl acetate recovery of thioglyolic acid from the sample, wherein thioglycolic acid is derivatised by methanol in the presence of concentrated sulphuric acid at temperature 80°C for 15 minutes, while the same is recovered from the sample by ethyl acetate by liquid-liquid micro extraction for 5 minutes with all the operations performed in the same container.
EFFECT: method enables increasing the sensitivity and accuracy, reducing the length of the analysis, simplifying the sample preparation, saving consumption of highly purified materials.
3 tbl, 1 ex, 2 dwg
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