DETERMINATION METHOD OF FORMALDEHYDE IN URINE WITH HELP OF GAS-CHROMATOGRAPHIC ANALYSIS Russian patent published in 2017 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: invention refers to the medicine, particularly, to the toxicological studies, and may be used for determination of a formaldehyde in urine. For this purpose, 0.1 cm³ of the 0.1% penta-fluoro-benzyl-hydroxyl amine (PFBHA) water solution is added to an urine sample with the volume of 1 cm³. The obtained solution is heated in a vial during 30 minutes at 60°C for the headspace analysis. Then, the gas-chromatographic analysis of the headspace is conducted on a capillary column with a flame-ionization detector. The content of a formaldehyde is determined according to a calibration curve.

EFFECT: invention of simplification of the sample preparation procedure, reducing of the analysis duration, reducing of the analyzed sample volume, reducing of costs for PFBHA reagent, and the possibility to conduct the gas-chromatographic analysis of the headspace instead of HPLC.

2 cl, 3 tbl, 3 dwg, 1 ex