DIFFERENTIAL DIAGNOSTIC TECHNIQUE FOR BRONCHIAL ASTHMA Russian patent published in 2013 - IPC G01N33/50 

FIELD: medicine.

SUBSTANCE: what is presented is a differential diagnostic technique for severe bronchial asthma, including defining the pulmonary ventilation function and analysing mononuclear cells recovered from patient's venous blood that is followed by calculation of an index characterising the inflammatory process. The monocytes are cultured into dendritic cells. A half of the cultures are added with N-athylcarboxamidoadenosine (NECA). The prepared dendritic cells are collected and analysed for the mRNA IL-8 content by polymerase chain reaction with reverse transcription. A probability P of referring the individual to a group of the patients suffering bronchial asthma is calculated by system of equations. If P>0.5, severe bronchial asthma is diagnosed.

EFFECT: invention enables differentiating severe bronchial asthma and other degrees of bronchial asthma.

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