METHOD FOR ASSESSING HYDROGEN PEROXIDE CONTENT IN TUMOUR CELLS AFTER EFFECT OF ANTICANCER PREPARATION Russian patent published in 2013 - IPC G01N33/52 

FIELD: medicine.

SUBSTANCE: invention may be used to assess the hydrogen peroxide (H₂O₂) content in tumour cells after the effect of an anticancer preparation, particularly cisplatin. The method is implemented as follows: the tumour cells representing a cell line of human cervical adenocarcinoma HeLa Kyoto are exposed to the effect of cisplatin in the concentration of 1.85-3.85 mcg/ml. The H₂O₂ intracellular content is measured by scanning laser microscopy to determine fluorescence signal intensity for sensor excitement at wavelengths 458 nm and 488 nm. The relation of fluorescence signal intensity at wave length 488 nm to fluorescence signal intensity at wave length 458 nm (F₄₈₈/F₄₅₈) is calculated and used to consider the hydrogen peroxide intracellular content. To assess the dynamic hydrogen peroxide content is ensured by determining fluorescence signal intensity in sensor excitement at wavelengths 458 nm and 488 nm. The relation of fluorescence signal intensity at wave length 488 nm to fluorescence signal intensity at wave length 458 nm is calculated and used to consider the hydrogen peroxide intracellular content, each 60 seconds for 30 minutes.

EFFECT: method enables localising the hydrogen peroxide source in the cell, and thereby considering the mechanisms of cisplatin toxic action at the molecular level, as well as provides eliminating the assessment error of the hydrogen peroxide content caused nu non-specific sensor accumulation.

3 ex, 1 dwg