FIELD: biotechnology.
SUBSTANCE: group of inventions relates to the field of biotechnology and is directed to nucleic acid molecules which encode a protein exhibiting properties of a biosensor for detection of hydrogen peroxide in living cells having fluorescence in the red region of the spectrum. The nucleic acid molecules are derived by method of genetic engineering. Protein for detection of hydrogen peroxide has the amino acid sequence shown in SEQ ID NO:2. The host cell and the expression cassette are also provided, comprising the said nucleic acid molecules.
EFFECT: use of the inventions enables to carry out microscopy in thick layers of tissues, and provides the possibility of joint microscopy of several fluorescent structures.
4 cl 7 dwg
| Title |
Year |
Author |
Number |
| ISOLATED NUCLEIC ACID CODING FLUORESCENT BIOSENSOR FOR DETECTION OF HYDROGEN PEROXIDE, EXPRESSION CASSETTE, CELL THAT PRODUCES BIOSENSOR, ISOLATED FLUORESCENT BIOSENSOR FOR DETECTION OF HYDROGEN PEROXIDE, ISOLATED NUCLEIC ACID THAT CODES FLUORESCENT BIOSENSOR, EFFICIENTLY FUSED WITH NUCLEIC ACID THAT CODES SIGNAL OF INTRACELLULAR LOCALISATION |
2011 |
- Bilan Dmitrij Sergeevich
- Belousov Vsevolod Vadimovich
- Luk'Janov Sergej Anatol'Evich
|
RU2493260C2 |
| CHIMERIC PROTEIN BEING FLUORESCENT BIOSENSOR FOR SIMULTANEOUS DETECTION OF HYDROGEN PEROXIDE AND PHOSPHATIDYL INOSITOL-3,4,5-TRIPHOSPHATE, NUCLEINIC ACID CODING SUCH PROTEIN, EXPRESSION CASSETTE AND EUCARIOTIC HOST CELL |
2012 |
- Belousov Vsevolod Vadimovich
- Mishina Natalija Mikhajlovna
- Chudakov Dmitrij Mikhajlovich
|
RU2498996C1 |
| MODIFIED BIOSENSOR FOR DETECTING INTRACELLULAR PH |
2014 |
- Belousov Vsevolod Vadimovich
- Matlashov Mikhail Egorovich
- Zagajnova Elena Vadimovna
- Ermakova Julija Gennadevna
- Lukjanov Sergej Anatolevich
|
RU2603060C2 |
| BIOSENSOR FOR DETECTION OF HYDROGEN PEROXIDE IN LIVE CELLS, POSSESSING HIGHER STABILITY TO pH CHANGE |
2009 |
- Belousov Vsevolod Vadimovich
|
RU2434943C2 |
| ISOLATED NUCLEIC ACID CODING FLUORESCENT BIOSENSOR, EXPRESSION CASSETTE, CELL, PRODUCING FLUORESCENT BIOSENSOR, ISOLATED FLUORESCENT BIOSENSOR |
2012 |
- Bilan Dmitrij Sergeevich
- Belousov Vsevolod Vadimovich
- Luk'Janov Sergej Anatol'Evich
|
RU2515903C2 |
| NUCLEIC ACID ENCODING FRET-BASED FAR-RED BIOSENSOR FOR INTRACELLULAR CASPASE 3 ACTIVITY MEASUREMENT |
2013 |
- Luk'Janov Konstantin Anatol'Evich
- Zlobovskaja Ol'Ga Anatol'Evna
|
RU2535981C1 |
| MODIFIED GENETICALLY CODED PHOTOSENSITIZER |
2013 |
- Sarkisyan Karen Sergeevich
- Mishin Aleksandr Sergeevich
- Lukyanov Sergej Anatolevich
|
RU2599443C2 |
| ISOLATED KillerRed PROTEIN-CODING NUCLEIC ACID (VERSIONS), ISOLATED PROTEIN (VERSIONS), EXPRESSION CASSETTE, CELL CONTAINING EXPRESSION CASSETTE |
2011 |
- Luk'Janov Konstantin Anatol'Evich
- Serebrovskaja Ekaterina Olegovna
- Luk'Janov Sergej Anatol'Evich
|
RU2458129C1 |
| ISOLATED NUCLEIC ACID, ENCODING OPERATIVELY FUSED INTRAMOLECULAR DIMER OF KillerRed PROTEIN, EXPRESSION CASSETTE, CELL, WHICH PRODUCES CHIMERIC PROTEIN AND CONTAINS EXPRESSION CASSETTE, ISOLATED CHIMERIC PROTEIN |
2011 |
- Luk'Janov Konstantin Anatol'Evich
- Serebrovskaja Ekaterina Olegovna
- Luk'Janov Sergej Anatol'Evich
|
RU2491342C1 |
| NOVEL FLUORESCENT PROTEINS FROM ENTACMAEA QUADRICOLOR AND METHOD OF OBTAINING SAID PROTEINS |
2007 |
- Luk'Janov Sergej Anatol'Evich
- Chudakov Dmitrij Mikhajlovich
|
RU2395581C2 |
