FIELD: chemistry.
SUBSTANCE: method for simultaneous determination of two analytes via bioluminescent molecular microanalysis involves treating the solid phase with a biospecific reagent, separating the unreacted liquid phase, treating the solid phase with an enzyme-containing conjugate, separating the liquid phase and the solid phase and analysing the solid phase, wherein the biospecific reagent for the first treatment of the solid phase is two immunoglobulins or one immunoglobulin with dual specificity or two oligonucleotides, and final treatment of the solid phase is carried out simultaneously with two enzyme-containing conjugates consisting of, respectively, recombinant Ca2+-dependent photoprotein obelins with different bioluminescence characteristics and molecules of different biospecificity, where the recombinant obelins used are obelin W92F; H22E and obelin Y138F, with subsequent analysis on a tablet bioluminometer with fast moving wideband colour filters with spectral and time separation of signals.
EFFECT: improved method.
3 ex, 4 dwg
