FIELD: biotechnology.
SUBSTANCE: vector plasmids ("docking vectors") are proposed for the synthesis of transgenes (RES), which represent a cloning vector, which is included in the cloning module consisting of four gene joints (GJ 1-4) and three nucleotide sequences (NS 1-3) located between them, where each of GJ comprises from 2 to 4 non-variable rare restriction sites from more than 6 nucleotides, and the nucleotide sequences between GJ include "inserts" which while cloning are replaced in the NS 1 by the promoter module, in the NS 2 by expression module and in NS 3 by 3'-regulatory module. At that, either GJ 1 or GJ 2 independently contain 3-4 non-variable rare restriction sites of more than 6 nucleotides. According to the invention, the variation of connection vector RES is also proposed which is designed for the multiple cloning (MC), and characterised in that at least one of the three NS is the module of multiple cloning with a site of multiple cloning comprising common restriction sites which are unique in the docking vector RES.
EFFECT: improvement of the method.
6 cl, 21 dwg
