MEMBRANE PROTEIN RENATURATION METHOD Russian patent published in 2013 - IPC C07K1/02 C12P21/00 

Abstract RU 2500684 C2

FIELD: biotechnologies.

SUBSTANCE: renatured membrane protein obtaining method is proposed. The above method involves production of a homogeneous solution containing a denatured membrane protein, a detergent mixture, phospholipide or phospholipide mixture and apolipoprotein or its equivalent with: further removal of detergents and formation of lipid-protein nanodiscs (LPND) containing renatured protein.

EFFECT: method provides production of renatured membrane proteins with high yield, which are built into LPND, which can be used at development of new medical products, biocatalysts, biosensors and biophotonic devices.

8 dwg, 2 ex

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RU 2 500 684 C2

Authors

Ljukmanova Ekaterina Nazymovna

Shenkarev Zakhar Olegovich

Kirpichnikov Mikhail Petrovich

Arsen'Ev Aleksandr Sergeevich

Dates

2013-12-10Published

2011-09-15Filed