FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions relates to the field of biotechnology. Claimed are: a method of two-phase obtaining of liposomes, a method of obtaining a diagnostic reagent for the determination of the prothrombin time and a method of obtaining a diagnostic reagent for the determination of the thromboplastin time. The method of two-phase obtaining of liposomes includes obtaining a liposome emulsion. The liposome emulsion with the concentration of lipids in it of 0.5-1.5 g/ml is obtained by mechanic mixing. The method of obtaining the diagnostic reagent for the determination of the prothrombin time includes application of liposome by the said method. The liposome emulsion and the natural or recombinant tissue factor are mixed in a proportion of 2:1 in a buffer medium. The method of obtaining the diagnostic reagent for the determination of the thromboplastin time includes application of liposome by the said method. The liposome emulsion and a negatively charged active component are mixed in a proportion of 1:1 in a buffer medium.
EFFECT: technical simplification of the method for obtaining liposome emulsions, simplicity of realisation of the methods for obtaining diagnostic objects is provided.
7 cl, 13 ex
