FIELD: biotechnologies.
SUBSTANCE: there described is a method for obtaining resident stem cells of mammal heart expressing surface markers c-kit or sca-I and/or MDR1, in the course of which the samples of myocard tissues are separated, ground, treated by collagenase and trypsin, cultivated at cultural bowl with coating from fibronectin by method of explant culture of ground samples with the following immunoselection.
EFFECT: invention allows obtaining enriched culture of resident stem cells of heart for the purposes of medicine and transplantology.
8 cl, 3 dwg, 4 ex
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