FIELD: medicine.
SUBSTANCE: method includes cultivation of pluripotent stem cells in culture medium, which does not contain substance, stimulating activation of canonical signal way Wnt, during period of time between beginning of differentiation induction and 24 hours before period of increased expression of genes of canonical Wnt. After that carried out is cultivation of pluripotent stem cells in culture medium, which contains substance, stimulating activation of canonical signal way Wnt, during period of time from 24 to 96 hours, starting from 24 to 0 hours before period of increased expression of genes of canonical Wnt.
EFFECT: method makes it possible to induce cardiomyocyte differentiation efficiently and selectively.
15 cl, 15 dwg, 6 ex
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