METHOD OF CULTURING MYOBLASTS IN VITRO FOR PRODUCING MYOBLAST BIOMASS FOR NUTRITION PURPOSES Russian patent published in 2014 - IPC C12N5/00 A23J1/02 

Abstract RU 2506309 C2

FIELD: chemistry.

SUBSTANCE: invention relates to cellular engineering, biotechnology and food industry. Disclosed is a method of culturing myoblasts in vitro to obtain myoblast biomass, where the cultured cells used are immotalised animal myoblasts; cells are grown using a culture medium with non-animal proteins, and also containing haemoglobin, wherein the cells are kept in a proliferative state using fibroblast and/or hepatocyte growth factors to form biomas, and differentiation of myoblasts into myocytes is then induced by removing fibroblast and/or hepatocyte growth factors from the medium, and in the case of cells with normal myostatin expression, a myostatin inhibitor is added to the medium.

EFFECT: method can be used in food industry to produce safe meat food products in large amounts over a short period of time.

6 cl, 3 tbl, 9 ex

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RU 2 506 309 C2

Authors

Fedorova Ekaterina Alekseevna

Dates

2014-02-10Published

2012-03-11Filed