FIELD: chemistry.
SUBSTANCE: invention relates to virology, particularly to culturing cells and tissue for producing and studying viruses. The strain of cells of the synovial membrane of a young pig is obtained by cultivating growing tissue explants of the synovial membrane of a young pig in a DMEM culture medium with 10% fetal bovine serum (FBS). The cell culture of the synovial membrane of a young pig is sensitive to viruses of classical swine fever, African swine fever, Aujeszky's disease and can be used to study viruses as well as in diagnostic studies. The strain of the synovial membrane of a young pig is stored in the collection of cell cultures of the National Research Institute for Veterinary Virology and Microbiology of Russia under No.65 and is deposited in the Special Collection of Grafted Somatic Cell Cultures of Agricultural and Industrial Animals RKKK(P) (SKHZH RASKHN) of the Y.R. Kovalenko National Research Institute for Experimental Veterinary (VIEV) under No.81.
EFFECT: obtaining strain of cells of the synovial membrane of a young pig having stable biological properties, which is suitable for virology and diagnostic studies, which is sensitive to causal viruses of swine diseases such as African and classical swine fever and Aujeszky's disease.
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Authors
Dates
2014-02-10—Published
2012-07-23—Filed