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2 140 451

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IPC

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Application

98111854/13, 1998-06-22

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Start date

1998-06-22

(22)

Actual filing date

1998-06-22

(45)

Published

1999-10-27

(72)

Inventor

Gusev A.A.Kuljashbekova Sh.K.Gerasimov V.N.Starov S.K.Khudjakov G.A.Fedorova E.E.Gerasimova N.I.Kozina A.A.

(73)

Holder

Vserossijskij Nauchno-Issledovatel'Skij Institut Zashchity Zhivotnykh

STRAIN OF CULTURED KIDNEY CELLS OF SIUS DOMESTICA FOR ANIMAL VIRUSES CULTURING Russian patent published in 1999 - IPC

Abstract RU 2140451 C1

FIELD: agriculture, veterinary science, virology. SUBSTANCE: invention relates to preparing antigens for diagnosis and prophylaxis of animal viral sicknesses. Monolayer-suspension strain of cultured cells SPEV-17/91 is obtained by treatment of the parent pig embryo renal cells culture with supermutagens, selection and adaptation to semisynthetic nutrient media. Obtained strain shows stable cultural and morphological properties. Strain shows sensitivity to foot-and-mouth virus, viruses VBS, BG, BA, EN, VES and plague KRS, it reproduces cells in monolayer and in suspension at titers 5.0-7.5 lg TCD₅₀. Strain is deposited in museum of cells VNIIZZH, deposited at 35-th passage in suspension in VSKK (SKHZH) RASKH at N 43. EFFECT: enhanced proliferative activity of the strain, prolonged safety of cellular layer, increased yield of cellular mass and virus-containing material, high infectious activity. 11 tbl, 15 ex

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RU 2 140 451 C1

Authors

Gusev A.A.

Kuljashbekova Sh.K.

Gerasimov V.N.

Starov S.K.

Khudjakov G.A.

Fedorova E.E.

Gerasimova N.I.

Kozina A.A.

Dates

1999-10-27—Published

1998-06-22—Filed