FIELD: biotechnologies.
SUBSTANCE: method provides for suspension of a biomass of cells till concentration is 15-30 wt % in the solution of polyvinyl alcohol with concentration of 6-9 wt %, which is prepared on a feed medium specific for growth of a certain phototrophic microorganism, freezing and storage of aliquots of the obtained suspension with the volume of 0.1-0.5 cm3 at -80÷-70°C.
EFFECT: method allows simplifying a cryopreservation process of cells of phototrophic microorganisms, increasing survival capacity of cells, providing this level of survival capacity at storage of cells during the period of up to a year and a half and using unfrozen specimens of cells as a concentrated seed grain.
13 ex
| Title | Year | Author | Number | 
|---|---|---|---|
| METHOD OF CRYOPRESERVATION OF MARINE MICROALGAE | 2012 | 
 | RU2496318C1 | 
| METHOD OF CRYOPRESERVATION OF CYANOBACTERIUM CELLS | 2017 | 
 | RU2650786C1 | 
| METHOD OF VITRIFICATION OF OVARIAL TISSUE | 2018 | 
 | RU2678106C2 | 
| METHOD FOR CRYOPRESERVATION OF BIOLOGICAL SAMPLES UNDER PRESSURE AND DEVICE FOR ITS IMPLEMENTATION | 2018 | 
 | RU2688331C1 | 
| METHOD FOR CRYOGENIC PRESERVATION OF CARP FISH SPERM | 2003 | 
 | RU2241324C2 | 
| METHOD OF CRYOPRESERVATION OF AXILLARY BUDS OF ASPEN PLANTS IN VITRO | 2013 | 
 | RU2565803C2 | 
| METHOD FOR PRESERVING VIABILITY OF HEMATOPOIETIC STEM CELLS OF UMBILICAL BLOOD AFTER WASHING FROM CRYOPROTECTANT | 2023 | 
 | RU2821586C1 | 
| METHOD OF STORAGE OF CELL CULTURES IN SUSPENSION | 2017 | 
 | RU2660075C1 | 
| PROTECTIVE MEDIUM FOR CRYOCONSERVATION OF STURGEON FISH SPERM | 2018 | 
 | RU2683682C1 | 
| BIOSENSOR BASED ON MICROALGAE CELLS FOR DETECTING HEAVY METALS AND HERBICIDES IN AQUEOUS SYSTEMS | 2009 | 
 | RU2426779C1 | 
Authors
Dates
2014-02-27—Published
2012-10-04—Filed