FIELD: biotechnologies.
SUBSTANCE: method provides for suspension of a biomass of cells till concentration is 15-30 wt % in the solution of polyvinyl alcohol with concentration of 6-9 wt %, which is prepared on a feed medium specific for growth of a certain phototrophic microorganism, freezing and storage of aliquots of the obtained suspension with the volume of 0.1-0.5 cm3 at -80÷-70°C.
EFFECT: method allows simplifying a cryopreservation process of cells of phototrophic microorganisms, increasing survival capacity of cells, providing this level of survival capacity at storage of cells during the period of up to a year and a half and using unfrozen specimens of cells as a concentrated seed grain.
13 ex
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Authors
Dates
2014-02-27—Published
2012-10-04—Filed