BIOSENSOR BASED ON MICROALGAE CELLS FOR DETECTING HEAVY METALS AND HERBICIDES IN AQUEOUS SYSTEMS Russian patent published in 2011 - IPC C12N1/12 C12N11/02 C12Q1/06 G01N33/18 

FIELD: chemistry.

SUBSTANCE: biosensor contains photo-autotrophic microalgae cells, the fluorescent characteristics of the photosynthesis system of which vary in the presence of cytotoxic chemical compounds in their surroundings: heavy metal ions and herbicides. Cells of green and diatomic microalgae are immobilised in cryogenic gel of polyvinyl alcohol: a cell suspension is deposited on a surface and the cells enter macropores of the polymer carrier under the effect of centrifugal force (5000-14000 g) for 1-10 minutes. A highly sensitive and stable biosensor is obtained based on components taken in the following ratio in wt %: microalgae cells 0.015-1.1; polyvinyl alcohol 7-15; aqueous phase - up to 100. Low concentrations of heavy metals and herbicides in aqueous systems are determined at flow rate of up to 360 ml/h based on the change in the value of relative variable fluorescence chlorophyll cells in the biosensor. The biosensor can be used for a maximum of 60 days.

EFFECT: high sensitivity of the biosensor.

2 dwg, 5 ex