FIELD: biotechnology.
SUBSTANCE: invention relates to a method for preserving viability of hematopoietic stem cells of umbilical blood after washing from cryoprotectant. Said method consists in defrosting umbilical blood concentrate with hematopoietic stem cells CD34+ (HSC); washing the hematopoietic stem cells from the cryoprotectant, centrifuging three times at 300 g for 10 minutes, removing the supernatant layer, resuspending the obtained precipitate to obtain washed cells, which are further brought to the required volume with a washing solution at a rate of not more than 20*10'8 leukocytes in 20 ml of said solution.
EFFECT: invention is effective for preserving viability of umbilical blood stem cells after washing from cryoprotectant.
1 cl, 1 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR REDUCING TOXICITY OF CRYOPRESERVATION SOLUTION BASED ON DIMETHYL SULFOXIDE AFTER THAWING OF HEMATOPOIETIC STEM CELLS | 2020 |
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RU2744614C1 |
| METHOD OF CRYOPRESERVATION OF HEMATOPOIETIC STEM CELLS OF UMBILICAL BLOOD | 2009 |
|
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| METHOD FOR WASHING CRYOPRESERVED MESENCHYMAL STROMAL CELLS | 2024 |
|
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| COMBINATION CRYOPROTECTOR "DIMETHYL SULPHOXIDE/RHEOPOLYGLUCIN" FOR STEM CELL CRYOPRESERVATION AND METHOD FOR CRYOPRESERVATION THEREOF FOR CLINICAL APPLICATIONS | 2014 |
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| BIOMEDICAL CELL PREPARATION | 2017 |
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| METHOD FOR CRYOPRESERVATION OF HAEMOPOIETIC UMBILICAL STEM CELLS | 2013 |
|
RU2547426C1 |
| METHOD FOR CRYOGENIC PRESERVATION OF HAEMATOPOIETIC UMBILICAL CORD STEM CELLS | 2012 |
|
RU2554405C2 |
| AUTOLOGIC HEMOPOIETIC STEM CELLS PREPARATION AND METHOD FOR PRODUCING, CRYOGENIC PRESERVING AND APPLYING IT IN TREATING TRAUMATIC NERVOUS SYSTEM DISEASE CASES | 2005 |
|
RU2283119C1 |
| METHOD FOR TRANSPLANTATION OF PERIPHERAL BLOOD AUTOLOGOUS HAEMOPOIETIC STEM CELLS | 2019 |
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RU2734121C1 |
| METHOD FOR ADRIFT FREEZING OF PERIPHERAL BLOOD PRECURSOR CELLS AT A TEMPERATURE OF MINUS 80 °C WITH A SOLUTION OF HEMODIOLITE | 2019 |
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RU2716574C1 |
