METHOD FOR PRESERVING VIABILITY OF HEMATOPOIETIC STEM CELLS OF UMBILICAL BLOOD AFTER WASHING FROM CRYOPROTECTANT Russian patent published in 2024 - IPC A01N1/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to a method for preserving viability of hematopoietic stem cells of umbilical blood after washing from cryoprotectant. Said method consists in defrosting umbilical blood concentrate with hematopoietic stem cells CD34+ (HSC); washing the hematopoietic stem cells from the cryoprotectant, centrifuging three times at 300 g for 10 minutes, removing the supernatant layer, resuspending the obtained precipitate to obtain washed cells, which are further brought to the required volume with a washing solution at a rate of not more than 20*10'8 leukocytes in 20 ml of said solution.

EFFECT: invention is effective for preserving viability of umbilical blood stem cells after washing from cryoprotectant.

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