FIELD: biotechnologies.
SUBSTANCE: invention refers to a method of species identification of L.casei/paracasei, L.fermentum, L.plantarum, L.rhamnosus lactobacilli. The proposed method involves performance of a PNR reaction with species-specific primers; besides, primers are built, which are specific to the first gene of operon FIFO ATP of synthase (a gene of subunit a) and a gene of uracylphosphoribosyltransferase, which precedes it, for L.casei/paracasei and L.rhamnosus and a gene of uracyltransport protein for L.plantarum and L. fermentum.
EFFECT: proposed solution can be used for identification of kinds of lactobacilli in different specimens of human and animal organisms, in pharmaceutical preparations and in food products.
2 cl, 1 dwg, 3 tbl
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