FIELD: chemistry.
SUBSTANCE: invention relates to biotechnology. The method of producing globotriose involves enzymatic transfer of a galactose monomer from a galactose donor to lactose acting as an acceptor. The galactose monomer donor used is β-galactosyl azide. The enzyme used is α-1,4-galactosyl synthase, which is obtained by introducing two substitutes D327G and P402D in corresponding positions of the amino acid sequence of the wild-type alpha-galactosidase gene isolated from Thermotoga maritima. The reaction is carried out at 37°C and pH 5.0 until complete splitting of β-galactosyl azide.
EFFECT: invention increases output and purity of globotriose.
2 cl, 2 dwg, 1 ex
