FIELD: chemistry.
SUBSTANCE: disclosed is a method for in vivo large-scale synthesis of sialylated oligosaccharides and a microorganism containing heterologous genes which code GlcNAc-synthetase, sialic acid synthase, ClcNAc-6-phosphate-2-epimerase and sialyl transferase, wherein endogenous genes which code sialic acid aldolase (NanA) and ManNac-kinase (NanK) were delegated or inactivated.
EFFECT: invention can be used in industrial synthesis of sialyl galactose.
58 cl, 4 tbl, 20 dwg, 17 ex
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