FIELD: biotechnologies.
SUBSTANCE: in the method they cultivate callus cultures from sterile explants of stem segments, leaves, leaf stakes. Basic nutrient media are used, such as Murasige-Skuga and Woody Plant Medium with content and ratio of growth regulators: 6-benzylaminopurine - 0.2-1.5 mg/l, α-naphthyl acetic acid - 0.2-0.5 mg/l, indolebutyric acid - 2 mg/l. Further adaptation is carried out with light of 2000 lux and nutrient mode of regenerants in a greenhouse with production of planting material for forest cultures.
EFFECT: method makes it possible to produce planting material with high inherited properties.
2 tbl
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METHOD OF MICROCLONAL REPRODUCTION AND OBTAINING PLANTING MATERIAL OF PLEASANT WEIGELA (WEIGELA SUAVIS (KOM) LHBAILEY) AND FLOWERING WEIGELA "VARIEGATA" (WEIGELA FLORIDA "VARIEGATA" BUNGE A DC) | 2016 |
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METHOD OF OBTAINING PLANT RAW MATERIALS OF THE SIBERIAN IRIS (IRIS SIBIRICA L.) BY BIOTECHNOLOGY METHODS | 2017 |
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METHOD OF GROWING PLANTING MATERIAL | 2011 |
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METHOD FOR INTRODUCTION INTO CULTURE IN VITRO, OBTAINING CALLUSES AND REGENERANT PLANTS OF SIBERIAN ALEXANDRIAN LAUREL (PHLOJODICARPUS SIBIRICUS (STEPH.) K.-POL.), USING VEGETATIVE AND GENERATIVE ORGANS AS PRIMARY EXPLANTS | 2021 |
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BREEDING OF PLUMEPOPPY CORDATE (MACLEAYA CORDATA (WILLD.) R.BR.) (VARIANTS) | 2003 |
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METHOD FOR CLONAL MICRO-PROPAGATION OF LILAC IN VITRO | 2010 |
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METHOD OF MICROCLONAL PROPAGATION OF MANCHURIAN KIRKAZONA (ARISTOLOCHIA MANSHURIENSIS KOM.) | 2023 |
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METHOD FOR GROWING PLANT RAW MATERIALS OF SIBERIAN IRIS (IRIS SIBIRICA L.) WITH A GIVEN CONTENT OF EXTRACTIVES | 2021 |
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METHOD FOR MICROCLONAL MULTIPLICATION OF LEAF BEGONIA | 2004 |
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RU2290786C2 |
Authors
Dates
2014-05-10—Published
2012-12-07—Filed