METHOD OF MICROCLONAL REPRODUCTION OF BLACK ALDER IN VITRO Russian patent published in 2014 - IPC A01H4/00 A01G7/00 A01G23/00 

Abstract RU 2515385 C1

FIELD: biotechnologies.

SUBSTANCE: in the method they cultivate callus cultures from sterile explants of stem segments, leaves, leaf stakes. Basic nutrient media are used, such as Murasige-Skuga and Woody Plant Medium with content and ratio of growth regulators: 6-benzylaminopurine - 0.2-1.5 mg/l, α-naphthyl acetic acid - 0.2-0.5 mg/l, indolebutyric acid - 2 mg/l. Further adaptation is carried out with light of 2000 lux and nutrient mode of regenerants in a greenhouse with production of planting material for forest cultures.

EFFECT: method makes it possible to produce planting material with high inherited properties.

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RU 2 515 385 C1

Authors

Sivolapov Vladimir Alekseevich

Sivolapov Aleksej Ivanovich

Tabatskaja Tat'Jana Mikhajlovna

Blagodarova Tat'Jana Aleksandrovna

Dates

2014-05-10Published

2012-12-07Filed