GRAPE MICROCLONAL PROPAGATION METHOD Russian patent published in 2025 - IPC A01G17/00 A01H4/00 

FIELD: agriculture.

SUBSTANCE: artificial culture medium for microclonal propagation of grapes has pH of 5.75 and contains components dissolved in distilled or deionised water in said concentrations, mg/l, macroelements: NH₄NO₃ 370-400; CaCl₂⋅2H₂O 89-96; Ca(NO₃)₂⋅4H₂O 460-471.26; KH₂PO₄ 170-180; K₂SO₄ 900-990; MgSO₄ 175-180.54; mesoelements: FeSO₄⋅7H₂O 27.5-32; Na₂EDTA⋅2H₂O 35-37.5; microelements: CuSO₄⋅5H₂O 0.2-0.25; H₃BO₃ 5.9-6.2; MnSO₄⋅H₂O 20-22.3; Na₂MoO₄⋅2H₂O 0.20-0.25; ZnSO₄⋅7H₂O 8-8.6; vitamins: myo-inositol 100; nicotinic acid 0.5; pyridoxine hydrochloride 0.5; thiamine hydrochloride 1; ascorbic acid 200-400; sucrose 30000; α-naphthylacetic acid 0.2; 6-benzylaminopurine 0.2; glycine 2-3; coal 2000; agar for solid media 6000. Method for microclonal propagation of grapes involves planting an isolated explant on an artificial nutrient medium for microclonal propagation of grapes, having pH of 5.75, containing components dissolved in distilled or deionised water in said concentrations, mg/l, macroelements: NH₄NO₃ 370-400; CaCl₂⋅2H₂O 89-96; Ca(NO₃)₂⋅4H₂O 460-471.26; KH₂PO₄ 170-180; K₂SO₄ 900-990; MgSO₄ 175-180.54; mesoelements: FeSO₄⋅7H₂O 27.5-32; Na₂EDTA⋅2H₂O 35-37.5; microelements: CuSO₄⋅5H₂O 0.2-0.25; H₃BO₃ 5.9-6.2; MnSO₄⋅H₂O 20-22.3; Na₂MoO₄⋅2H₂O 0.20-0.25; ZnSO₄⋅7H₂O 8-8.6; vitamins: myo-inositol 100; nicotinic acid 0.5; pyridoxine hydrochloride 0.5; thiamine hydrochloride 1; ascorbic acid 200-400; sucrose 30000; α-naphthylacetic acid 0.2; 6-benzylaminopurine 0.2; glycine 2-3; coal 2000; agar for solid media 6000 with subsequent cultivation until additional buds and roots appear.

EFFECT: inventions make it possible to carry out fast rooting and growth of grapes of elite varieties, to obtain a healthy plant of elite varieties ready for acclimatization in 1-2 months of culture in vitro.

4 cl, 5 dwg, 2 tbl, 3 ex