YEAST TRANSFORMANT YARROWIA LIPOLYTICA PRODUCING CANTHAXANTHIN Russian patent published in 2024 - IPC C12N1/19 C12N15/81 C12P23/00 

FIELD: biotechnology.

SUBSTANCE: disclosed is a yeast transformant Yarrowia lipolytica, which produces the carotenoid canthaxanthin and contains at least one copy of each of the following genes in the chromosome: M. circinelloides CarB gene coding phytoendehydrogenase, M. circinelloides CarRP gene coding bifunctional enzyme phytoene synthase/lycopene β-cyclase, Y. lipolytica HMGR1 gene coding 3-hydroxy-3-methylglutaryl-coenzyme A reductase enzyme, Y. lipolytica ERG20 gene coding farnesyl pyrophosphate synthase, Y. lipolytica ERG12 gene coding mevalonate kinase, nucleotide sequence CarRP-GGPPs7 coding polypeptide, obtained by combining bifunctional enzyme phytoene synthase/lycopene β-cyclase carRP M. circinelloides and geranylgeranyl pyrophosphate synthase GGPPs7 Synechococcus sp., nucleotide sequence CarRP-ERG20 (F88C) coding polypeptide, obtained by combining M. circinelloides carRP and a modified version of farnesyl pyrophosphate synthase ERG20^F88C Y. Lipolytica, Haematococcus pluvialis codon-optimized HPBKT gene, which codes β-carotene ketolase, the nucleotide sequence of which is given in the sequence list under the number SEQ ID NO: l, Schizosaccharomyces pombe codon-optimized gene SpHXKl, coding hexokinase, the nucleotide sequence of which is given in the sequence list under the number SEQ ID NO: 2, codon-optimized gene YHT1 Y. lipolytica, coding hexose transporter, nucleotide sequence of which is given in sequence list under number SEQ ID NO: 3, codon-optimized gene YHT3 Y. lipolytica, coding hexose transporter, nucleotide sequence of which is given in sequence list under number SEQ ID NO: 5, codon-optimized gene YHT4 Y. lipolytica, coding hexose transporter, nucleotide sequence of which is given in the sequence list under the number SEQ ID NO: 7. Also disclosed is a recombinant yeast strain Y. lipolytica VKPM Y-5144—a producer of canthaxanthin.

EFFECT: invention widens the range of microorganisms producing the carotenoid canthaxanthin.

2 cl, 5 dwg, 5 tbl, 3 ex