HIGHLY SENSITIVE METHOD OF DETERMINING IMMUNOGLOBULIN-PROTEASE ACTIVITY USING POLYMER MATRICES Russian patent published in 2014 - IPC G01N33/573 G01N33/543 

FIELD: biotechnology.

SUBSTANCE: in the wells of a polystyrene plate the polymeric matrices of nonprotein nature are sorbed - DNA, chitin, and then to the wells IgG and the solution containing proteolytic enzymes specific to this matrix are added. After incubation, during which the proteolytic cleavage of immunoglobulin molecules is carried out, the conjugate of enzyme peroxidase with protein A of staphylococcus and the substrate of this enzyme are added to the wells. Then the recording of the results of the reaction is carried out using a vertical beam spectrophotometer by measuring light absorbance at 492 nm and calculation of IgG-proteinase activity according to amount of hydrolysed substrate of the enzymatic reaction.

EFFECT: method has high sensitivity, lack of need to use protein antigens, low flow of substrate at which the reaction itself and its detection is performed in the same well of a microplate.

1 dwg, 1 tbl, 12 ex