METHOD OF DETERMINING IgG PROTEINASE ACTIVITY Russian patent published in 2009 - IPC G01N33/53 G01N33/573 

FIELD: chemistry; biochemistry.

SUBSTANCE: invention relates to biochemistry and can be used in enzymology, microbiology, pharmacology, clinical biochemistry for screening and quantitative evaluation of activity of proteolytic enzymes. The method is realised as follows: immunoglobulin G (IgG) is sorbed in cavities of a microplate, a solution is then placed in cavities of the microplate, containing proteolytic enzymes. Incubation is done, resulting in splitting of immunoglobulin molecules and their desorption. Contents of the cavities are removed. The conjugate of the enzyme with protein A staphylococcus, a substrate of this enzyme, is added and incubation is repeated. The result of this reaction is analysed with a spectrometre with a vertical beam by measuring absorption of light at 492 nm and activity of IgG-proteinase is calculated from the amount of hydrolysed substrate of the enzymic reaction.

EFFECT: design of a method which is simple to implement, provides for good reproducibility of results, has high sensitivity, does not require use bacteria antigens, in which there is low consumption of substrate, possibility of using immunoglobulins regardless of their specificity.

1 tbl, 6 ex