METHOD OF DETERMINING IgA-PROTEINASE ACTIVITY Russian patent published in 2011 - IPC G01N33/53 G01N33/573 G01N33/543 

FIELD: medicine.

SUBSTANCE: immunoglobulin-A is sorbed in microplate wells, then after incubation and treatment, a solution containing a proteolytic enzyme is introduced in the microtablet wells. It is followed with incubation; the contents of the wells are poured out, and then an enzyme conjugate, e.g. peroxidase, with immunoglobulin-A Fc-fragment antibodies and a substratum of this enzyme are introduced. After incubation of a reaction mixture, reaction results are accounted by a spectrophotometre. IgA-protease activity is calculated by the amount of hydrolysed substratum of the enzymatic reaction.

EFFECT: use of the invention allows facilitating determination of the IgA-protease activity level, the method exhibits good result reproducibility, high sensitivity, low substratum consumption and the absence of necessity to use of immunoglobulins of certain specificity.

1 dwg, 1 tbl, 4 ex