FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology. Recombinant tuberculous vaccine is proposed that contains the first antigen, which is a purified recombinant Ag85A protein of Mycobacterium tuberculosis (M. tuberculosis), the second antigen, which is a hybrid recombinant ESAT6-CFP10 protein of M. tuberculosis, and an adjuvant comprising a first water-soluble high molecular dextran polysaccharide for immobilizing the Ag85A and ESAT6-CFP10 proteins of said antigens, an unmethylated CpG oligonucleotide with a phosphorothioate bond, and the second water-soluble high molecular dextran polysaccharide, other than the first water-soluble high molecular dextran polysaccharide, to immobilize said CpG oligonucleotide.
EFFECT: invention makes it possible to equalize the kinetics of interaction of individual components of the vaccine with immunocompetent cells, which increases the effectiveness of both primary and booster vaccinations using the proposed vaccine.
12 cl, 6 dwg, 1 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| POLYANTIGENIC VACCINE FOR PREVENTING AND ADJUNCTIVE TREATMENT OF TUBERCULOSIS | 2019 |
|
RU2724896C1 |
| RECOMBINANT PLASMID pESAT6-CFP10-DBD, RECOMBINANT STRAIN Escherichia coli M15 [pREP4, pESAT6-CFP10-DBD], METHOD FOR PREPARING, IMMOBILISING, CONCENTRATING AND PURIFYING RECOMBINANT PROTEIN ESAT6-CFP10-DBD ON DEXTRAN, RECOMBINANT PROTEIN ESAT6-CFP10-DBD AND IMMUNOGENIC COMPOSITION CONTAINING PROTEIN ESAT6-CFP10-DBD | 2013 |
|
RU2539026C1 |
| METHOD FOR OBTAINING IMMUNOGENIC COMPOSITION BASED ON Ag85A-DBD HYBRID PROTEIN AND DEXTRANE; pAg85A-DBD RECOMBINANT PLASMIDE; Escherichia coli [pREP4, pAg85A-DBD] STRAIN; Ag85A-DBD CHIMERIC PROTEIN | 2013 |
|
RU2520078C1 |
| METHOD FOR OBTAINING IMMUNOGENIC COMPOSITION BASED ON CFP10-DBD HYBRID PROTEIN AND DEXTRANE; pCFP10-DBD RECOMBINANT PLASMIDE; Escherichia coli [pREP4, pCFP10-DBD] STRAIN; CFP10-DBD CHIMERIC PROTEIN AND THEIR APPLICATION | 2013 |
|
RU2546875C1 |
| METHOD OF PRODUCTION OF IMMUNOGENIC COMPOSITION BASED ON FUSION PROTEIN pESAT6-DBD AND DEXTRAN, RECOMBINANT PLASMID pESAT6-DBD, STRAIN OF Escherichia coli, CHIMERIC PROTEIN ESAT6-DBD AND THEIR APPLICATION | 2013 |
|
RU2520737C1 |
| RECOMBINANT PLASMID, Escherichia coli STRAIN, CHIMERIC PROTEIN AND THEIR APPLICATION | 2010 |
|
RU2422525C1 |
| RECOMBINANT STRAIN MYCOBACTERIUM BOVIS rBCG-1 BASED ON VACCINE STRAIN MYCOBACTERIUM BOVIS BCG STRAIN RUSSIA 368 | 2022 |
|
RU2807732C1 |
| RECOMBINANT PLASMID, Escherichia coli STRAIN, CHIMERIC PROTEIN AND THEIR APPLICATION | 2010 |
|
RU2422524C1 |
| RECOMBINANT PLASMID pAg85A-CBD, Escherichia coli [pREP4, pAg85A-CBD] STRAIN, CHIMERIC PROTEIN Ag85A-CBD AND THEIR APPLICATION | 2010 |
|
RU2429292C1 |
| HYBRID PROTEIN CFP10-ESAT6, INDUCING REACTION OF HYPERSENSITIVITY OF DELAYED TYPE WITH RESPECT TO M.Tuberculosis, CODING IT CHIMERIC NUCLEIC ACID AND RECOMBINANT PLASMID EXPRESSION VECTOR, CONTAINING IT, METHOD OF OBTAINING HYBRID PROTEIN AND BASED ON IT DOSED MEDICINAL FORM FOR INTRACUTANEOUS INJECTION | 2008 |
|
RU2360926C9 |
