METHOD OF PRODUCING FATTY ACID METHYL ESTERS USING LIPASE MIXTURE (VERSIONS) Russian patent published in 2014 - IPC C12P7/64 C12N9/20 C07C67/00 

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. Disclosed are versions of a method of producing fatty acid methyl esters (biodiesel) in a solvent-free microwater system. The method involves step-by-step addition of methanol to triglycerides in the presence of a lipase preparation and carrying out a reaction in suitable conditions until the triglycerides are converted to fatty acid methyl esters. The lipase preparation contains at least two lipases, separately or together immobilised on a porous hydrophobic support, where one of the lipases has high affinity for partial glycerides and the other is position-specific to the sn-1,3 position, and a third optional lipase has high selectivity for the glycerine sn-2 position. The support is selected from a group comprising porous hydrophobic supports based on aliphatic or aromatic polymers. Also disclosed is a method of preparing a mixture of lipases immobilised on said support. The mixture contains Candida antarctica B lipase and at least one lipase obtained from Pseudomonas sp., Alcaligenes sp., Burkholderia sp. and Thermomyces lanuginosa.

EFFECT: lipase preparations have high resistance to hydrophilic substrates, demonstrate synergism between immobilised lipases and facilitate a high degree of conversion over a short period of time.

18 cl, 4 dwg, 5 tbl, 12 ex