FIELD: biotechnology.
SUBSTANCE: method of purifying follicle-stimulating hormone (FSH) is described. Immunoaffinity chromatography (IAC) is performed, followed by hydrophobic interaction chromatography (HIC), and then anion exchange chromatography (AEC). In the IAC step, elution is carried out using an elution solution with a pH of 7 to 8.
EFFECT: invention ensures the production of recombinant FSH with high yield and purity.
20 cl, 6 tbl, 2 dwg
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