METHOD OF ASSESSMENT OF ACTIVITY OF THERAPEUTIC AND PROPHYLACTIC PREPARATIONS AGAINST NATURAL SMALLPOX VIRUS Russian patent published in 2014 - IPC A61K35/76 C12N7/00 A61P31/12 

FIELD: biotechnology.

SUBSTANCE: method of assessment of activity of therapeutic and prophylactic preparations against natural smallpox comprises administering to an animal model of control and test groups in a predetermined scheme of suspension of the antiviral preparation under study, their intranasal infection with a strain of natural smallpox virus, incubation of the virus in animal bodies and determining the concentration of virus in the lungs of animals with the subsequent calculation of estimate values of decrease in the concentration of virus in lungs. The preparation is administered to animal bodies one day prior to infecting, on the day of infecting and every day during the time of the virus incubation. The animal models are used as outbred white mice ICR of different sexes weighing 7-9 g. The strain of natural smallpox virus is used as the strain India-3a deposited at the National Collection of pathogens of viral infections and rickettsioses of the Federal budget institution of science State Research Centre of Virology and Biotechnology "Vector" under the registration number V-45.

EFFECT: adequate representation of human natural smallpox disease with use of highly virulent strain of natural smallpox virus and animal model.

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