METHOD FOR PREPARING INFECTIOUS BOVINE RHINOTRACHEITIS VACCINE Russian patent published in 2014 - IPC A61K39/265 C12N7/00 

FIELD: medicine.

SUBSTANCE: invention refers to veterinary microbiology, virology and biotechnology, and concerns a method for preparing an infectious bovine rhinotracheitis vaccine. The described method: preparing a virus material of the infectious bovine rhinotracheitis virus strain, infecting a continuous cell culture with the virus material, culturing the infectious bovine rhinotracheitis virus, collecting a virus fluid, inactivating it to prepare a fluid end product. The virus fluid is inactivated with an antioxidant solution prepared by electrolysis of 10.0-20.0% sodium chloride; electrolysis is continued until reaching pH 7.0-8.0, antioxidant concentration 0.7-0.9% and redox potential +1000±50 mV and an inactivator consumption 4.5-5.0 cm³ per 0.8-1.0 l of the virus fluid; the virus fluid inactivation is one-staged with the active chlorine concentration of Cac=200-300 mg/l for 60-70 min and at a temperature of 37-38°C at pH 7.2-7.4.

EFFECT: presented invention can be used in developing agents for specific prevention, and particularly for preparing the infectious bovine rhinotracheitis vaccine.

2 cl, 4 ex