FIELD: chemistry.
SUBSTANCE: method of obtaining yeasts extract, which contains biologically active highly-polymer RNA from dry baking yeasts Saccharomyces cerevisiae includes yeasts suspension in a water solution of sodium oleate, boiling the suspension with periodic mixing, centrifuging of the cooled to room temperature lysate, bringing the volume of the yeasts extract to the standard with distilled water with further separation of highly-polymer RNA from it, its addition to ointment or pouring into vials in a dose of 2-4 ml with further freezing and lyophilisation under specified conditions.
EFFECT: method makes it possible to increase the output of highly-polymer RNA.
1 ex, 1 tbl
Title | Year | Author | Number |
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METHOD OF PRODUCING PROTEIN-FREE BIOLOGICALLY ACTIVE HIGH-POLYMERIC RNA PREPARATION FROM DRY BAKER'S YEAST Saccharomyces cerevisiae | 2010 |
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HIGH-POLYMER RNA FROM BAKER'S YEAST, FREE FROM IMPURITY ASSOCIATIONS OF RNA WITH PROTEINS AND POLYSACCHARIDES | 2009 |
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Authors
Dates
2014-07-20—Published
2012-11-07—Filed