METHOD OF PRODUCING HIGH-POLYMERIC RNA FROM DRY BAKER'S YEAST Russian patent published in 2010 - IPC C12P19/34 C12N15/10 C12N1/18 

FIELD: chemistry; biochemistry.

SUBSTANCE: invention relates to biotechnology, in particular to extraction of physiologically active compounds and can be used in medicine. Dry yeast is suspended in a 1-2% aqueous solution of oleic acid titrated with an alkali to pH 7-8. The suspension is kept at 99-102°C for 40-60 minutes. Lysate cooled to 35-45°C is centrifuged without cooling. The supernatant fluid is discharged and NaCl is added to the discharged fluid until achieving concentration of 2-3 M. The suspension is kept for 20-24 hours at room temperature and centrifuged without cooling. Low-polymeric RNA is extracted from the obtained supernatant and the precipitate-extraction cake is washed with two portions of 2-3 M solution of NaCl and with four portions of 92-97% ethanol via successive resuspension at room temperature and centrifuging. High-polymeric RNA is extracted from the obtained extraction cake using distilled water and then dried using a conventional method. Ethanol is reclaimed through distillation. Natural oligoribonucleotides are extracted from the low-polymeric RNA through gel filtration on Sepharose-4B.

EFFECT: invention enables to obtain high-polymeric RNA with considerably high molecular weight as a result of using dry baker's yeast without preliminary soaking or using high temperature.

5 cl, 1 dwg, 1 ex