FIELD: medicine.
SUBSTANCE: what is offered is a method for producing high-polymer yeast RNA of used beer yeast. Used beer yeast concentrated by centrifugation is suspended in an aqueous solution of oleic acid titrated by alkali to pH 7-8. The suspension is kept at 98-102°C for 40-60 minutes. The hot lysate is settled for 20-24 h at room temperature. A supernatant is poured out with using a siphon. Then NaCl is added to the poured out liquid to the concentration 2-3 M. The suspension is kept for 20-96 h at room temperature. Then it is centrifuged without cooling in a low-speed centrifuge. The prepared cakes are consistently washed out in the NaCl solution 2-3 M and 92-96% ethanol by resuspension at room temperature and low-speed centrifugation.
EFFECT: invention promotes spread-out of the raw-material base and simplification of the high-polymer RNA technology.
1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PRODUCING HIGH-POLYMERIC RNA FROM DRY BAKER'S YEAST | 2009 |
|
RU2403288C1 |
| METHOD OF PRODUCING AMPHIPHILIC HIGHLY POLYMERIC RNA FROM DRY YEAST | 2022 |
|
RU2801533C1 |
| METHOD OF HIGH-POLYMERIC RNA MANUFACTURE FROM YEAST | 2008 |
|
RU2392329C1 |
| AMPHIPHILIC HIGH-POLYMER RNA FROM BAKER'S YEAST | 2009 |
|
RU2430968C2 |
| METHOD OF HIGH-POLYMERIC RNA MANUFACTURE FROM YEAST | 2008 |
|
RU2392328C2 |
| SIMPLE METHOD OF EXTRACTING HIGHLY-POLYMER RNA FROM YEASTS | 2012 |
|
RU2522900C2 |
| METHOD OF PRODUCING PROTEIN-FREE BIOLOGICALLY ACTIVE HIGH-POLYMERIC RNA PREPARATION FROM DRY BAKER'S YEAST Saccharomyces cerevisiae | 2010 |
|
RU2510854C2 |
| HIGH-POLYMER RNA FROM BAKER'S YEAST, FREE FROM IMPURITY ASSOCIATIONS OF RNA WITH PROTEINS AND POLYSACCHARIDES | 2009 |
|
RU2430969C2 |
| ANTIVIRAL AGENT | 2010 |
|
RU2436583C1 |
| METHOD FOR OBTAINING TOTAL RNA FROM BIOMASS OF SACCHAROMYCES CEREVISIAE YEAST CELLS | 2022 |
|
RU2781832C1 |
