STABLE PROBIOTIC GRANULES AND THEIR PRODUCTION METHOD Russian patent published in 2014 - IPC A23L1/30 A23K1/16 C12N1/18 

FIELD: food industry.

SUBSTANCE: invention relates to a probiotic composition used in the field of food industry and public health service and/or veterinary. The granulation method involves a stage of introduction of a probiotic composition (A) into the nutritional mixture intended for corresponding application, the probiotic containing probiotic yeast in an amount of 2 - 30 wt % of the total composition weight and a nutritional additive in an amount of 70 - 98 wt % of the total weight of the composition containing at least one ingredient chosen from vitamins, microelements, amino acids and other additives intended for usage in food industry or in the field of public health service and/or veterinary. Dry substances content (DM) in the probiotic yeast is equal to 93 - 98%; the mean diameter (d) of balls is equal to 800 - 1200 mcm. Then one performs water steam pumping at a temperature of 60°C - 85°C under a pressure of 0.5 - 4 bars into the mixture produced at the previous stage. Then one performs granulation by way of expression at a temperature of 70 - 92°C till production of granules with a diameter (D) equal to 2 - 6 mm and cooling. The draw die granulation temperature (T), the granules diameter (D), dry substances content (DM) and the balls mean diameter (d) are determined so that to prove the following equation: X=-196,482-[0,023×d]+[2,256×(DM)]-[14,793×T]+[3,046×D]+[6,25×10^-5×d×(DM)]+[0,001×d×T]+[5,63×10^-5×d×T]+[0,167×(DM)×T]-[0,036×(DM)×T]-[0,023×D×T]+[4,06×10^-6×d²]+[0,003×T²], wherein yeast losses after granulation X (log CFU/g of initial yeast - log CFU/g after granulation) are equal to less than 1 log CFU/g of the granulated product. By the proposed method one produces a probiotic granulate containing 0.01 - 5 wt % of probiotic yeast and 95 - 99.9 wt % of the corresponding nutritional mixture.

EFFECT: invention allows to adjust the total characteristics of probiotic yeast as well as the method parameters so that microorganisms losses after granulation are equal to less than 1 log CFU/g of the granulated product.

10 cl, 6 tbl