METHOD FOR MEASURING ANTIOXIDANT ACTIVITY OF BIOLOGICAL FLUIDS Russian patent published in 2014 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: method involves preparing an aqueous solution of equal molarity of quinone/hydroquinone pair of mediators in a phosphate buffer with pH=7.40, adding an analysed sample in the form of a biological fluid - blood plasma or serum to the pair of mediators and immersing an auxiliary electrode made of a carbon-based material and a silver chloride reference electrode into the prepared solution of the mediator with the added analysed sample; wherein a working electrode made of platinum is processed in sodium sulphate 0.1 mole/l by means of 60 cycles of a cyclic scanning of the potential within the range of 500 to -600 mV at a scanning rate of the potential of 750 mV/s, and that is followed by 15 scanning cycles within the range of 200 to 350 mV at a scanning rate of the potential of 750 mV/s; thereafter the said electrode is first immersed into the prepared solution of the pair of mediators, and 6 cycles of the cyclic scanning of the potential within the range of -600 to 800 mV at a scanning rate of the potential of 500 mV/s follow; the above solution is added with the analysed sample of the biological fluid in a ratio 1:1, and 6 cycles of the cyclic scanning of the potential within the range of -600 to 800 mV at a scanning rate of the potential of 500 mV/s are performed; the antioxidant concentration in the sample is assessed by a difference of current peaks on a cathode lead of cyclic voltamperograms recorded prior to and after the analysed sample is added to the solution of the pair of mediators in the form of the biological fluid.

EFFECT: higher measurement accuracy and reliability.

3 cl, 3 ex