METHOD OF PROPAGATION OF CYMBIDIUM in vitro Russian patent published in 2014 - IPC A01H4/00 

FIELD: biotechnology.

SUBSTANCE: invention is a method of propagation of cymbidium in vitro, comprising introduction of sterile explants in culture in vitro, micropropagation by separating the pseudobulbs from explant and planting on the Murashige and Skoog nutrient medium, rooting of pseudobulbs in the ground, where, after the introduction in the culture in vitro the formed pseudobulbs are separated from the explant and transferred to the Murashige and Skoog agarised nutrient medium containing 30 g/l sucrose and epibrassinolide preparation solution in a concentration of 0.001 mg/l, then the pseudobulbs having root-shoots are separated from the explants, treated with the indoleacetic acid solution in a concentration of 1.0 mg/l for 15 minutes and rooted under ex vitro conditions in the ground with addition of moss and wood bark.

EFFECT: invention enables to increase the yield of propagated material due to increasing the amount of the formed pseudobulbs, with the positive impact of optimal concentrations of growth regulators.

3 tbl