NUTRIENT MEDIUM FOR CLONAL MICRO-PROPAGATION OF LILIES (LILIUM L.), IN VITRO CONDITIONS Russian patent published in 2021 - IPC C12N1/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. The invention is an original nutrient medium for efficient clonal micropropagation of lilies based on environment Quoirin-Lepoivre medium (QL) with half concentration of macro salts (NH₄NO₃ 200.0 mg/l; KNO₃ 900.0 mg/l; Ca(NO₃)₂ 600.0 mg/l; MgSO₄×7H₂O 180.0 mg/l; KH₂PO₄ 135.0 mg/l; H₃BO₃ 6.2 mg/l; MnSO₄×4H₂O 1.0 mg/l; ZnSO₄×7H₂O 8.6 mg/l; KI 0.08 mg/l; Na₂MoO₄×2H₂O 0.25 mg/l; CuSO₄×5H₂O 0.025 mg/l; CoCl₂×6H₂O 0.025 mg/l; FeSO₄×7H₂O 27.8 mg/l) containing EDTA, 37.3 mg/l; meso inositis 100.0 mg/l; thiamine chloride 0.4 mg /l; pyridoxine chloride 0.5 mg / l; 6-BAP 0.25 mg/l; isopropyl formic acid 0.1 mg/l, glucose 20000.0 mg/l; agar 8000.0 mg /l and distilled water up to 1 liter of the medium.

EFFECT: invention makes it possible to obtain 100% regeneration of explants of lily squame and as a result, in comparison with the traditionally used medium, to increase the reproduction coefficient more than twice.

1 cl, 2 dwg, 1 ex