FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, specifically to recombinant production of proteins, and can be used for production of interleukin-36 receptor antagonist. Bacterial strain E.coli BL21Star [DE3] (pET-IL36Raf) - producer of full-size human interleukin-36 receptor antagonist is obtained by transformation of cells of E.coli parental BL21Star [DE3] with plasmid vector pET-IL36Raf. Bacterial strain E.coli BL21Star [DE3] (pET-IL36Raf-His) - producer of full-size human interleukin-36 receptor antagonist with C-end polyhistidine tag is obtained by transformation of cells of E.coli parental BL21Star [DE3] with plasmid vector pET-IL36Raf-His.
EFFECT: higher efficiency of recombinant production of IL-36Ra.
6 cl, 4 dwg, 1 tbl, 3 ex
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